Article ID Journal Published Year Pages File Type
5419664 Progress in Nuclear Magnetic Resonance Spectroscopy 2011 30 Pages PDF
Abstract
► Introduction of a lanthanide ion into a protein leads to paramagnetic effects and partial alignment. ► Paramagnetic Relaxation Enhancements (PREs), Residual Dipolar Couplings (RDCs), and Pseudo-Contact Shifts (PCSs), among others, can be measured. ► Amplitude of paramagnetic effects depends on metal ions, protein size, and magnetic field strength. ► All paramagnetic effects and effects resulting from partial alignment are reviewed. ► Practical aspects include overview of tags used and characteristics of metal ions.
Related Topics
Physical Sciences and Engineering Chemistry Physical and Theoretical Chemistry
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