Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5420590 | Solid State Nuclear Magnetic Resonance | 2010 | 4 Pages |
Abstract
Conformational changes have been studied in silk fibers from the domestic silkworm Bombyx mori and a wild silkworm Samia cynthia ricini as a result of fractured by stretching. About 300 samples consisting of only the fractured regions of [1-13C]Ala or [1-13C]Gly labeled silk fibers were collected and observed by 13C CP/MAS NMR spectra. The total amount of these fractured fibers is only about 1Â mg and therefore we used a home-built 1Â mm microcoil MAS NMR probehead. A very small increase in the fraction of random coil was noted for the alanine regions of both silk fibroins and for the glycine region of B. mori silk fibroin. However, there is no difference in the spectra before and after fractured for the glycine region of S. c. ricini silk fibroin. Thus, the influence of fracture occurs exclusively at the Ala region for S. c. ricini. The relationship between sequence, fracture and structure is discussed.
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Physical Sciences and Engineering
Chemistry
Physical and Theoretical Chemistry
Authors
Kazuo Yamauchi, Shizuo Yamasaki, Rui Takahashi, Tetsuo Asakura,