Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5420928 | Solid State Nuclear Magnetic Resonance | 2006 | 8 Pages |
Abstract
The recently reported CSA-amplified PASS experiment correlates the spinning sidebands at the true spinning frequency Ïr with the spinning sidebands that would be obtained at the effective spinning frequency Ïr/N, where N is termed the scaling factor. The experiment is useful for the measurement of small chemical shift anisotropies, for which slow magic-angle spinning frequencies, required to measure several spinning sidebands, can be unstable. We have experimentally evaluated the reliability of this experiment for this application. In particular we have demonstrated that large scaling factors of the order of N=27 may be used, whilst still obtaining accurate chemical shift sideband intensities at the effective spinning frequency from the F1 projection. Moreover, the sideband intensities are accurately obtained even in the presence of significant pulse imperfections. A second application of the CSA-amplified PASS experiment is the measurement of the chemical shift anisotropy of sites that experience homonuclear dipolar coupling, as may be found in uniformly labelled biological molecules, or for nuclei with a high natural abundance. The effects of homonuclear dipolar coupling on CSA-amplified PASS spectra has been investigated by numerical simulations and are demonstrated using uniformly 13C enriched l-histidine monohydrochloride monohydrate.
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Physical Sciences and Engineering
Chemistry
Physical and Theoretical Chemistry
Authors
Robin M. Orr, Melinda J. Duer,