Development of long-term antimicrobial poly(methyl methacrylate) by incorporating mesoporous silica nanocarriers

•Mesoporous silica nanoparticle (MSN) loading in poly(methyl methacrylate) (PMMA) changed the mechanical properties depending on the incorporated concentration.•An anti-adherent effect against Candida albicans and Streptococcus oralis was observed without cytotoxicity in MSN-incorporated PMMA.•A long-term anti-microbial effect was observed over 2 weeks due to the slow release of amphotericin B after loading them into MSN-incorporated PMMA.

ObjectivePoly(methyl methacrylate) (PMMA) used as removable denture bases or orthodontic appliances has relatively poor antimicrobial properties, which accelerate oral infection and induce unfavorable odors. Mesoporous silica nanoparticles (MSNs) have been highlighted as a potential additive to overcome this issue because of their drug-loading capacity. Here, we present the long-term antimicrobial effect of MSN-incorporated PMMA with drug-loading capacity.MethodsAfter the MSNs were characterized, MSN incorporation into chemically activated PMMA (0.5, 1, 2.5 or 5 wt%) relative to the methyl methacrylate powder by mass was fabricated into a rectangular specimen (1.4 × 3.0 × 19.0 mm) for a 3-point flexural test at a speed of 1 mm/min or a disk (∅ = 11.5 mm and d = 1.5 mm) for investigation of its antimicrobial effects.ResultsA typical spherical morphology with a well-ordered mesoporous structure of the MSNs was visualized and is beneficial for loading drugs and combining in matrixes. Among the tested levels of MSN incorporation in PMMA (0.5, 1, 2.5 or 5 wt%), only 5 wt% decreased the flexural strength (p < 0.05), whereas the flexural modulus was not significantly decreased (p > 0.05). The surface roughness and surface energy were increased with 2.5 wt% or 5 wt% incorporation. An anti-adherent effect against Candida albicans and Streptococcus oralis after 1 h of attachment was only observed with 2.5 and 5 wt% incorporation compared to a lack of MSNs (p < 0.05). A long-term antimicrobial effect was observed for 2 weeks with 2.5 wt% MSN-incorporated PMMA when amphotericin B was loaded into the MSNs on the PMMA surface.SignificanceThe long-term antimicrobial performance after loading amphotericin B into the MSN-incorporated PMMA suggests the potential clinical usefulness of MSN-incorporated PMMA resin.

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