Proliferation, behavior, and differentiation of osteoblasts on surfaces of different microroughness

•Role of different roughness components in the healing after implantation is scarce.•Surfaces with similar micronscale roughness might differ in spatial roughness.•The role of different roughness components in osseointegration must be studied.•Surfaces differing in micronscale roughness - not in other parameters - were used.•Surfaces with Ra parameters of about 1-2 μm are optimal for osteblasts.

ObjectivesTitanium surface roughness is recognized as an important parameter influencing osseointegration. However, studies concerning the effect of well-defined surface topographies of titanium surfaces on osteoblasts have been limited in scope. In the present study we have investigated how Ti surfaces of different micrometer-scale roughness influence proliferation, migration, and differentiation of osteoblasts in-vitro.MethodsTitanium replicas with surface roughnesses (Ra) of approximately 0, 1, 2, and 4 μm were produced and MG-63 osteoblasts were cultured on these surfaces for up to 5 days. The effect of surface micrometer-scale roughness on proliferation, migration in time-lapse microscopy experiments, as well as the expression of alkaline phosphatase, osteocalcin, vascular-endothelial growth factor (VEGF), osteoprotegerin (OPG), and receptor activator of nuclear factor kappa-B ligand (RANKL) were investigated.ResultsProliferation of MG-63 cells was found to decrease gradually with increasing surface roughness. However, the highest expression of alkaline phosphatase, osteocalcin and VEGF was observed on surfaces with Ra values of approximately 1 and 2 μm. Further increase in surface roughness resulted in decreased expression of all investigated parameters. The cell migration speed measured in time-lapse microscopy experiments was significantly lower on surfaces with a Ra value of about 4 μm, compared to those with lower roughness. No significant effect of surface roughness on the expression of OPG and RANKL was observed.SignificanceThus, surfaces with intermediate Ra roughness values of 1-2 μm seem to be optimal for osteoblast differentiation. Neither proliferation nor differentiation of osteoblasts appears to be supported by surfaces with higher or lower Ra values.

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