Bio-functionalizing heterogeneous phase activated titanium by multiphoton ionization energy mechanism to harmonize cell proliferative behavior

•Generating heterogeneous titanium oxide phase surface by ultra-short pulsed laser interaction technique.•Modulating laser pulse interaction time vary oxide phase concentrations present in phase transformed titanium surface.•The approach empowers site specific mammalian cell adhesion and migration due to cocktail oxide phases.•To develop smart titanium based biomedical devices such as implants, biosensors and cell sorting applications.

Cellular interactions are regulated by various mechanical, physical and chemical factors that are either introduced to or are pre-existing in their local microenvironments. These factors include geometric confinement, cell-substrate interactions and cell-cell contacts. The systematic elucidation of these dictating mechanisms is crucial for fundamental understanding of regenerative medicine and for designing biomedical devices. Here, we have developed an elegant multi-photon ionization based mechanism, which accomplishes selective surface bio-functionalization of native titanium substrates, to achieve stable cellular confinements. In particular, we applied selective titanium phase activation for cellular confinement of mouse fibroblasts and osteoblast cells in an effort to examine their directionality and proliferative behavior under confinement. The experimental results suggest, both mouse fibroblasts and osteoblasts can be manipulated, guided and aligned along an induced orientation by selective hongquiite phase activation. The cell viability of both fibroblast and osteoblast cells were observed through fluorescent assays and SEM techniques. The phase activated surface fabricated influenced both nuclei and actin cytoskeletal re-arrangement of cell structures.