A facile and simple polyaniline-poly(ethylene oxide) based glucose biosensor

•Glucose biosensor produced with nanofibers of PAni-PEO in dispersion.•Simple and efficient biosensor without the need for complex and expensive materials.•Parameters were optimized to produce an efficient and reproducible biosensor.•Fast amperometric response, selectivity and wide linear range were obtained.

In this work, the fabrication and performance of a simple and efficient glucose biosensor based on chemically synthesized polyaniline-poly(ethylene oxide) nanocomposite (PAni-PEO) have been demonstrated. The nanocomposite was synthesized through rapid-mixing polymerization and characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and Fourier transform infrared spectroscopy (FT-IR). The nanocomposite and glucose oxidase (GOx) solutions were placed on a platinum electrode by drop-casting, to produce a glucose biosensor that was characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), and chronoamperometry. Under optimal conditions, the biosensor showed a linear range from 1 to 10 mM (R2 = 0.990) with a high sensitivity of 16.04 μA mM−1 cm−2 and a detection limit of 0.82 mM. The interference from ascorbic acid (AA) and uric acid (UA) was also investigated. The results indicated that the biosensor exhibited a satisfactory linear range, sensibility, and selectivity for use as a glucose biosensor, considering the normal range of approximately 4-6 mM of glucose concentration in human blood.