Use of pre-wetted lightweight fine expanded shale aggregates as internal nutrient reservoirs for microorganisms in bio-mineralized mortar

Interest in developing bio-based self-healing cement-based materials has gained broader attention in the concrete community. One of challenges in developing bio-based self-healing cement-based materials is that cell death or insufficient metabolic activity might occur when the cells are inoculated to the cement paste. This paper investigates the use of internal nutrient reservoirs via pre-wetted lightweight fine expanded shale aggregates to improve cell viability in mortar. Incorporation of internal nutrient reservoirs resulted in an increase in the vegetative cells remaining without any substantial loss in strength. These results pave the way to develop a self-healing and self-curing concrete with an extended service life.