Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5449002 | Optics Communications | 2017 | 4 Pages |
Abstract
We develop a point-spread function (PSF) engineering approach to imaging the spatial and spectral information of molecular emissions using a spatial light modulator (SLM). We show that a dispersive grating pattern imposed upon the emission reveals spectral information. We also propose a deconvolution model that allows the decoupling of the spectral and 3D spatial information in engineered PSFs. The work is readily applicable to single-molecule measurements and fluorescent microscopy.
Related Topics
Physical Sciences and Engineering
Materials Science
Electronic, Optical and Magnetic Materials
Authors
Sravan Munagavalasa, Bryce Schroeder, Xuanwen Hua, Shu Jia,