| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5469650 | Procedia CIRP | 2017 | 4 Pages |
Abstract
To improve on attrition rates during drug development due to nephrotoxicity, a better in vitro model of the kidney is needed. We demonstrate a model of the kidney proximal tubule in which a sheet of proximal tubule epithelial cells is fixed as a membrane separating two fluid chambers without the presence of a supporting membrane. Cell sheets were prepared using a Poly(N-isopropylacrylamide) (PIPAAm) temperature responsive surface and a bespoke bioreactor was constructed using a 3D printer to produce an appropriate mould. Cell sheets were suspended using a fibronectin functionalized surface seeded with cells. Further testing is needed to determine viability in bioreactor.
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Authors
William Loewenhardt, Rachel Saunders, Rachel Lennon, Brian Derby,