Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5478299 | Algal Research | 2017 | 11 Pages |
Abstract
Microalgal cultivation systems for energy production are not yet economically viable due to the low yield of lipids and biomass. In this study, we propose a chemical approach, using exogenous bioactive molecules for improving algal growth, lipid content and the production of high-value alpha-linolenic acid (ALA), using Chlorella protothecoides as a model. Based on our findings, among the cytokinin and auxin classes, Kinetin increased the biosynthesis of ALA by 26.5% at 1 ppm concentration, and indole-3-butyric acid at 2 ppm improved the biomass and lipid productivity up to 29.15 ± 2.02 g mâ 2 dayâ 1, and 6.69 ± 0.56 g mâ 2 dayâ 1, respectively. Methyl jasmonate as a stress-related phytohormone also showed a positive stimulatory effect on growth of C. protothecoides, and the biomass productivity was increased up to 25.26 ± 1.48 g mâ 2 dayâ 1 at the concentration of 5 ppm (lipid content: 219.66 ± 13.20 mg gâ 1 dw; lipid productivity: 5.61 ± 0.25 g mâ 2 dayâ 1; ALA: 0.81 ± 0.16 g mâ 2 dayâ 1). A difference in the composition of fatty acids, due to the type and concentrations of stress-related phytohormones was observed, and under the influence of stress-related phytohormones, the biosynthesis of saturated fatty acids increased, and polyunsaturated fatty acids decreased in C. protothecoides. Butylated hydroxyanisole, at a concentration of 0.05 ppm, increased the ALA content by 60.5%. Our study shows that the proposed bioactive molecules could increase the biomass productivity of C. protothecoides at a lower cost compared to the use of acetate as a carbon source.
Related Topics
Physical Sciences and Engineering
Energy
Renewable Energy, Sustainability and the Environment
Authors
Ali Parsaeimehr, Elena I. Mancera-Andrade, Felipe Robledo-Padilla, Hafiz M.N. Iqbal, Roberto Parra-Saldivar,