Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5478335 | Algal Research | 2017 | 13 Pages |
Abstract
We investigated the simultaneous effects of light and temperature on pigments, lipid remodeling, and omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA) partitioning into lipid classes of P. lutheri. Biomass was produced in batch cultivation using high bicarbonate supply, and hence, any potential accumulation of lipid and triacylglycerols (TAG) containing n-3 LC-PUFA was triggered by nitrogen (N) limitation. The maximum productivities of both eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids were obtained after nitrate-depletion when P. lutheri was cultivated at optimal growth conditions; these occurred at an intermediate temperature of 18 °C and low light intensity (40 μmol photons·mâ 2·sâ 1) for EPA, but there was no impact of light (40 or 200 μmol photons·mâ 2·sâ 1) for DHA production. Under these respective optimum conditions, TAG accounted for 82-84% of TFA on day 15 of cultivation, which contained up to 76-84% of the total cellular EPA and 67-81% of total cellular DHA. Our results clearly demonstrate the process of lipid remodeling in P. lutheri and, for the first time, a potential membrane lipid turnover with transfer of n-3 LC-PUFA (EPA and DHA) from membranes (polar lipids, PL) to storage lipids (TAG), highlighting the accumulation of n-3 LC-PUFA-rich oil during N-starvation (i.e., TAG containing: EPA ~ 12-13% and DHA ~ 5-6% of TFA). When cultivated under low light conditions, P. lutheri additionally accumulated substantial quantities of antioxidant pigments (i.e., fucoxanthin, diadinoxanthin and β-carotene), which add value to extracted bioactive oil for functional food applications. Omega-3 rich oil accumulation and pigment levels in P. lutheri appear to be simultaneously regulated by both light and temperature, in addition to N-limitation during batch-cultivation.
Keywords
EPASFAMUFATChlTFAChlGC-FIDn-3 LC-PUFATCARAFDWPDATomega-3 long-chain polyunsaturated fatty acidsash-free dry weightROSβ-carβ-caroteneEicosapentaenoic aciddocosahexaenoic acidsaturated fatty acidsmonounsaturated fatty acidsstandard deviationTriacylglycerolsTAG یا triacylglycerols DHADiatoxanthinDiadinoxanthinChlorophyllphospholipid:diacylglycerol acyltransferaseFucoxanthinPolar lipidsfatty acid methyl estersFAME یا fatty acid methyl esters NitrogenPavlova lutheriTotal fatty acidsTotal carotenoidsGas Chromatography-Flame Ionization DetectorReactive oxygen species
Related Topics
Physical Sciences and Engineering
Energy
Renewable Energy, Sustainability and the Environment
Authors
Freddy Guihéneuf, Dagmar B. Stengel,