Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5485795 | Ultrasound in Medicine & Biology | 2016 | 9 Pages |
Abstract
Coronary intervention for myocardial infarction often results in microvascular embolization of thrombus. Sonoreperfusion therapy (SRP) using ultrasound and microbubbles restored perfusion in our in vitro flow model of microvascular obstruction. In this study, we assessed SRP efficacy using whole blood as the perfusate with and without tissue plasminogen activator (tPA). In a phantom vessel bearing a 40-μm-pore mesh to simulate the microvasculature, microthrombi were injected to cause microvascular obstruction and were treated using SRP. Without tPA, the lytic rate increased from 2.6 ± 1.5 mmHg/min with 1000-cycle pulses to 7.3 ± 3.2 mmHg/min with 5000-cycle ultrasound pulses (p < 0.01). The lytic index was similar for tPA-only ([2.0 ± 0.5] Ã 10â3 mmHgâ1 minâ1) and 5000 cycles without tPA ([2.3 ± 0.5] Ã 10â3 mmHgâ1 minâ1) (p = 0.5) but increased ([3.6 ± 0.8] Ã 10â3 mmHgâ1 minâ1) with tPA in conjunction with 5000-cycles ultrasound (p < 0.01). In conclusion, SRP restored microvascular perfusion in whole blood, SRP lytic rate in experiments without tPA increased with ultrasound pulse length and efficacy increased with the addition of tPA.
Keywords
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Authors
Sebastiaan T. Roos, François T. Yu, Otto Kamp, Xucai Chen, Flordeliza S. Villanueva, John J. Pacella,