Reactive gamma-ketoaldehydes as novel activators of hepatic stellate cells in vitro

Article ID	Journal	Published Year	Pages	File Type
5501998	Free Radical Biology and Medicine	2017	39 Pages	PDF

Abstract

IsoLGs represent a newly identified class of activators of HSC in vitro, which are biologically active at concentrations as low as 500 pM, and are particularly effective at promoting a pro-inflammatory response and autophagy.

Keywords

XBP1 GAPDH ICAM-1 NF-kB ERK SfM qRT-PCR DAPI HBSS MTS 5-bromo-2-deoxyuridine UPR IL8 PARP IL6 TIMP-1 LC3B p38MAPK COL1A1 Collagen type I alpha 1 carboxy-H2DCFDA Isolevuglandins isolevuglandin PDI α-SMA Jnk 4-HNE CCL2 ATF4 2′,7′-dichlorodihydrofluorescein diacetate 4′,6-diamidino-2-phenylindole 4-hydroxy-2-nonenal BiP c-Jun N-terminal kinase IL1β ROS alpha smooth muscle actin Autophagy Endoplasmic reticulum stress inflammation LOX Interleukin-8 interleukin-6 Interleukin-1 beta BrdU analysis of variance ANOVA Enzyme-linked immunosorbent assay ELISA Oxidative stress CHOP standard error of the mean quantitative reverse transcription PCR serum free medium endoplasmic reticulum nuclear factor kappa B activating transcription factor 4 Fibrosis lactate dehydrogenase LDH Lysyl oxidase Hank's balanced salt solution SEM intracellular adhesion molecule Unfolded protein response X-box binding protein 1 Immunoglobulin heavy chain binding protein protein disulfide isomerase CCAAT-enhancer-binding protein homologous protein p38 mitogen-activated protein kinases poly (ADP-ribose) polymerase complete medium Chloroquine chemokine (C-C motif) ligand 2 glyceraldehyde 3-phosphate dehydrogenase Reactive oxygen species

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Reactive gamma-ketoaldehydes as novel activators of hepatic stellate cells in vitro

Authors

Lisa Longato, Fausto Andreola, Sean S. Davies, Jackson L. Roberts, Giuseppe Fusai, Massimo Pinzani, Kevin Moore, Krista Rombouts,