| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5502202 | Free Radical Biology and Medicine | 2017 | 4 Pages |
Abstract
â¢A robust assay for SOD activity is presented.â¢The assay is suitable for SOD measurements on a large number of samples.â¢Caveats are described to avoid pitfalls.
One of the most convenient methods for measuring superoxide dismutase activity is a plate assay using xanthine oxidase and the water soluble tetrazolium WST-1. For reliable results with WST-1, certain aspects of the procedure need to be adhered to. This article describes an appropriate protocol that minimizes sources of variability.
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Authors
Alexander V. Peskin, Christine C. Winterbourn,