Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5503713 | Mechanisms of Ageing and Development | 2017 | 51 Pages |
Abstract
We analyzed B cells infiltrating the Visceral Adipose Tissue (VAT) of young and old mice to identify contributors to the phenotypic and functional changes observed in aged B cells. We found higher percentages of pro-inflammatory (age-associated B cells, ABC) and less Follicular (FO) B cells in VAT as compared to spleen. VAT B cells express higher pro-adipogenic and inflammatory markers, including NF-kB, as compared to splenic B cells, and also secrete IgG2c antibodies, some of which have been shown to be autoimmune in other studies. Moreover, we found that in the presence of an adipocyte-conditioned medium FO B cells differentiated into ABC. Additional results showed production of pro-inflammatory chemokines by the adipocytes, suggesting a mechanism for B cell attraction by the VAT. These results are the first to show a direct effect of adipocytes on the generation of pro-inflammatory B cells.
Keywords
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Biochemistry, Genetics and Molecular Biology
Ageing
Authors
Daniela Frasca, Alain Diaz, Maria Romero, Thomas Vazquez, Bonnie B. Blomberg,