In silico characterization of Myogenic Factor 6 transcript of Hilsa, Tenualosa ilisha and putative role of its SNPs with differential growth

This study aimed at elucidating the structure of deduced protein Myf6 in an anadromous fish, T. ilisha (Hilsa) and polymorphism present in it among different fish species. Myf6 in Hilsa was identified from muscle Transcriptome sequences through Illumina HiSeq sequencing and designated as TiMyf6, the complete length was 1009 nt, in which ORF (open reading frame) consisted of 720 nt. Its secondary structure of modeled protein consisted of 8 helices, 6 helix-helix interacts, 32 beta turns and 26 gamma turns. The protein-protein interaction prediction of TiMyf6 protein showed functional association with 10 proteins. A total of 3 SNPs were identified in the slow and fast growing fish groups, which had altered the amino acids E/G, Q/G and V/A. These 3 SNPs identified in present study, may function as candidate marker to differentiate slow and fast growing species.