Evaluation of nephroblastoma overexpressed gene, a transcriptional target of forkhead box protein O1 in type 2 diabetes mellitus

•FOXO1 and CCN3 expression have a crucial role in type 2 DM.•FOXO1 and CCN3 didn't differentiate between complicated and uncomplicated type 2 DM.•Significant correlation was found between expression of FOXO1 and CCN3.

BackgroundType 2 diabetes mellitus (DM) is a chronic metabolic disease. The aim of this study was to evaluate the expression of FOXO1 and its target CCN3 in patients with type 2 DM in a trial to explore the molecular mechanism underlying β cell failure and to correlate the relationship between the two gene expressions, to each other, to the different clinico-pathological factors and to complications of T2DM.Study designThe expression of FOXO1 and CCN3 genes was evaluated by quantitative real time polymerase chain reaction (qPCR) in blood of 60 diabetics and 20 control.ResultsA high significant correlation was found between the studied groups regarding fold change of FOXO1 and CCN3 expression (P < 0.001). There was significant correlation between FOXO1 and CCN3 expression and many of the anthropometric measures or clinico-pathological factors among the studied groups.ConclusionThe results demonstrated the crucial role of FOXO1 and CCN3 in type 2 DM.