Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5519336 | Toxicon | 2017 | 7 Pages |
â¢A new member of the contryphan family, contryphan-Bt was isolated from the venom of Conus betilinus.â¢The sequence of contryphan-Bt is ascertained as ZSGCO(D-W)KPWC-NH2 (Z, pyroglutamic acid).â¢This finding is the first instance of the identification of pyroglutamic acid in contryphan.
A new member of the contryphans family was isolated from the venom of Conus betilinus, a vermivorous species distributed in the South China Sea. Its sequence, ZSGCO(D-W)KPWC-NH2 (Z, pyroglutamic acid), was established by a combination of de novo MS/MS sequencing and venom-duct transcriptome sequencing. The occurrence of D-Trp6 was confirmed by chemical synthesis and HPLC behavior comparison. Like known contryphans, contryphan-Bt produces the “stiff-tail” syndrome in mice and contains one disulfide bond, a hydroxyproline, a D-tryptophan, and an amidated C-terminus. However, contryphan-Bt differs from previously identified contryphans by a pyroglutamic acid at the N terminus. CD spectrum reveals that contryphan-Bt possess β-turn in solution.