Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5525204 | Cancer Letters | 2017 | 6 Pages |
â¢Screening with a panel of inhibitors against kinases identifies CDK inhibitor dinaciclib as a potential inhibitor for T-ALL.â¢The CDK inhibitor dinaciclib reduces cell growth and induces apoptosis.â¢Dinaciclib reduces colony number and size in semisolid medium.â¢Dinaciclib potentially blocks expression of pro-survival genes and induces cell cycle arrest.â¢Dinaciclib extends survival of mice in a T-ALL xenograft model.
T-cell acute lymphoblastic leukemia (T-ALL) is a heterogeneous disease of the blood affecting children, adolescents and adults. Although current treatment protocols for T-ALL have improved overall survival, a portion of T-ALL patients still experiences treatment failure. Thus, the development of novel therapies is needed. In this study, we used several patient-derived T-ALL cell lines to screen for an effective drug for T-ALL. Using a panel of 378 inhibitors against different kinases, we identified the CDK inhibitor dinaciclib as a potential drug for T-ALL. Dinaciclib treatment significantly reduced cell viability and completely blocked colony formation. Furthermore, cells treated with dinaciclib showed decreased expression of several pro-survival proteins including survivin, cyclin T1 and c-MYC. Dinaciclib treatment also increased accumulation of cells in G2/M phase and significantly induced apoptosis. Finally, dinaciclib extended survival of mice in a T-ALL cell xenograft model. Collectively, these data suggest that the CDK inhibitor dinaciclib is an active drug for T-ALL in the preclinical settings.