| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5530766 | Cellular Immunology | 2017 | 5 Pages |
â¢HIV infected individuals sourced PBMCs were used in this study.â¢The evaluation was based on in vitro experiment.â¢It is a straightforward and time-efficient method for evaluating vaccines.â¢Ad5-HIVgag vaccine can stimulate HIV-specific PBMCs in HIV infected individuals.
A novel method for HIV vaccine efficacy evaluation was established and the experimental conditions optimized. This novel method was then applied to determine whether a recombinant adenovirus type 5 HIV therapeutic vaccine expressing Gag antigen (Ad5-HIVgag) could stimulate HIV-specific cellular responses in vitro. The results indicated that HIV-specific IFN-gama production lymphocytes were induced by the Ad5-HIVgag vaccine. Compared with other methods, this in vitro stimulation method is safe and time-efficient, and the result is more intuitive. It has the potential to be regarded as a supplement to other methods for evaluating the IFN-gamma production by PBMCs to HIV vaccination.
