| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5533077 | Journal of Molecular Biology | 2017 | 4 Pages |
â¢High content imaging-based kinome siRNA and inhibitor screens were performed.â¢Aurora kinase B was the top hit for H3.3S31 phosphorylation in both screens.â¢Aurora kinase B phosphorylates nucleosomal H3.3 at S31 in vitro.
Serine 31 of histone H3.3 is phosphorylated during mitosis in mammalian cells and has been shown to influence chromosome segregation. We established a screening system to identify candidate kinase(s) phosphorylating H3.3S31 by using siRNA library targeting 720 human kinases, and we confirmed the results with an independent kinase inhibitor screen. Aurora kinase B was the best candidate hit in both screens. Moreover, the kinase activity of Aurora B on H3.3S31 was biochemically confirmed with nucleosomal substrates in vitro.
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