Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5534263 | Molecular and Cellular Endocrinology | 2017 | 9 Pages |
â¢Estradiol treatment influences myocardial glucose and free fatty acid metabolism in rats fed a high fat (HF) diet.â¢In vivo estradiol treatment impacts on cardiac iNOS expression/activity in HF fed rats.â¢Estradiol acts via Akt to attenuate the detrimental effects of cardiac iNOS in HF fed rats.
The aim of this study was to investigate the in vivo effects of 17β-estradiol (E2) on myocardial metabolism and inducible nitric oxide synthase (iNOS) expression/activity in obese rats. Male Wistar rats were fed with a normal or a high fat (HF) diet (42% fat) for 10 weeks. Half of the HF fed rats were treated with a single dose of E2 while the other half were placebo-treated. 24 h after treatment animals were sacrificed. E2 reduced cardiac free fatty acid (FFA) (p < 0.05), L-arginine (p < 0.01), iNOS mRNA (p < 0.01), and protein (p < 0.05) levels and translocation of the FFA transporter (CD36) (p < 0.01) to the plasma membrane (PM) in HF fed rats. In contrast, Akt phosphorylation at Thr308 (p < 0.05) and translocation of the glucose transporter GLUT4 (p < 0.05) to the PM increased after E2 treatment in HF rats. Our results indicate that E2 acts via the PI3K/Akt signalling pathway to partially protect myocardial metabolism by attenuating the detrimental effects of increased iNOS expression/activity in HF fed rats.
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