Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5540030 | Developmental & Comparative Immunology | 2017 | 44 Pages |
Abstract
As the teleost specific immunoglobulin, IgT plays important roles in systemic and mucosal immunity. In the current study, in rainbow trout, we have cloned the heavy chain (IgÏ) genes of a secretory form of IgT2 as well as the membrane and secretory forms of a third IgT subclass, termed IgT3. Conserved cysteine and tryptophan residues that are crucial for the folding of the immunoglobulin domain as well as hydrophobic and hydrophilic residues within CART motif were identified in all IgT subclasses. Through analysis of the rainbow trout genome assembly, IgÏ3 gene was found localized upstream of IgÏ1 gene, while IgÏ2 gene situated on another scaffold. At the transcriptional level, IgÏ1 was mainly expressed in both systemic and mucosal lymphoid tissues, while IgÏ2 was largely expressed in systemic lymphoid organs. After LPS and poly (I:C) treatment, IgÏ1 and IgÏ2 genes exhibited different expression profiles. Interestingly the transcriptional level of IgÏ3 was negligible, although its protein product could be identified in trout serum. Importantly, a previously reported monoclonal antibody directed against trout IgT1 was able to recognize IgT2 and IgT3. These data demonstrate that there exist three subclasses of IgT in rainbow trout, and that their heavy chain genes display different expression patterns during stimulation. Overall, our data reflect the diversity and complexity of immunoglobulin in trout, thus provide a better understanding of the IgT system in the immune response of teleost fish.
Keywords
LLRV domainJ segmentC domainjoining segmentRAGIgHRSSVLRBCRqPCRIGTmAbMonoclonal antibodyimmunoglobulin IGLrecombination signal sequenceleucine-rich repeatConstant domainVariable domainlight chain variable domainheavy chain variable domainimmunoglobulin light chainImmunoglobulin heavy chainSubclassMass spectrometryRainbow troutRacequantitative real-time PCRliquid chromatographyB cell receptorVariable lymphocyte receptor
Related Topics
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Biochemistry, Genetics and Molecular Biology
Developmental Biology
Authors
Nu Zhang, Xu-Jie Zhang, Dan-Dan Chen, J. Oriol Sunyer, Yong-An Zhang,