Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5540145 | Developmental & Comparative Immunology | 2017 | 27 Pages |
Abstract
Neuropeptide Y (NPY) plays different roles in mammals such as: regulate food intake, memory retention, cardiovascular functions, and anxiety. It has also been shown in the modulation of chemotaxis, T lymphocyte differentiation, and leukocyte migration. In fish, NPY expression and functions have been studied but its immunomodulatory role remains undescribed. This study confirmed the expression and synthesis of NPY in S. salar under inflammation, and validated a commercial antibody for NPY detection in teleost. Additionally, immunomodulatory effects of NPY were assayed in vitro and in vivo. Phagocytosis and superoxide anion production in leukocytes and SHK cells were induced under stimulation with a synthetic peptide. IL-8 mRNA was selectively and strongly induced in the spleen, head kidney, and isolated cells, after in vivo challenge with NPY. All together suggest that NPY is expressed in immune tissues and modulates the immune response in teleost fish.
Keywords
PYYNBTDPIPAMPsNPYDAPIMS-222FCSIDCfMLPN-formyl-methionyl-leucyl-phenylalanineLPSPBSL15SHK-1Peptide Ynatural killer4′6-diamidino-2-phenylindoleBSAcAMPDMSOPMAROSnitroblue tetrazoliumCyclic adenosine monophosphatebovine serum albuminpathogen-associated molecular patternsinterleukinELISAEnzyme-linked immunosorbent assayImmunomodulationbase pairsDimethyl sulfoxideSalmo salarfetal calf serumDendritic cellImmature dendritic cellCytokinesPhagocytosisphorbol myristate acetatephosphate buffer salinelipopolysaccharidePoly I:Cpolyinosinic-polycytidylic acidpeptide YYHead kidneyReactive oxygen speciesNeuropeptide Y
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Authors
Roxana González-Stegmaier, Franz Villarroel-EspÃndola, René ManrÃquez, Mauricio López, Mónica Monrás, Jaime Figueroa, Ricardo EnrÃquez, Alex Romero,