Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5552942 | Biomedicine & Pharmacotherapy | 2017 | 8 Pages |
Abstract
Multiple sclerosis is the most common autoimmune inflammatory and demyelinating disease of the central nervous system. The experimental autoimmune encephalomyelitis (EAE) is an appropriate and a well-establish model for studying the pathogenesis of MS. β-caryophyllene (BCP), a natural sesquiterpene found in many plant species, is a potent anti-inflammatory compound. Herein we investigated the in vitro and in vivo immunomodulatory effects of BCP on C57BL/6 mice induced with EAE. BCP was in vitro evaluated (4, 20, and 40 μM) on splenocytes obtained from EAE-induced C57BL/6 mice, and in vivo (25 or 50 mg/kg/day) orally administered on EAE-mice. The clinical course, body weight, cytokines and oxygen radicals production were investigated in C57BL/6 EAE-mice. In vitro and in vivo immunological responses were evaluated by ELISA, and CNS sections were stained by hematoxylin and eosin methods The in vitro production of H2O2, NO, IFN-γ, and TNF- α was inhibited by BCP (20 and 40 μM) in cultured cells from EAE-mice. BCP (25 and 50 mg/kg/day) reduced clinical score and severity of EAE and inhibited H2O2, NO, TNF-α, IFN-γ and, IL-17 production. EAE-mice, orally treated with BCP (mainly at 50 mg/kg/day), displayed levels of cytokines and clinical signs similar to animals with no EAE disease, demonstrating the therapeutic action of BCP on EAE animals. Histopathological and histomorphometric analysis confirmed that BCP treatment significantly reduced the numbers of inflammatory infiltrates and attenuated neurological damages in the CNS of EAE-mice.
Keywords
NF-κBCB2IFN-γConAβ-CaryophylleneEAEiNOSBCPConcanavalin ACFAcomplete Freund's adjuvantHydrogen peroxideexperimental autoimmune encephalomyelitistumor necrosis factor-alphaCNScentral nervous systemTNF-αnuclear factor kappa BNatural productsMultiple sclerosisnitrite oxidenitric oxide synthaseH2O2Interferon gammacannabinoid receptor type 2
Related Topics
Health Sciences
Medicine and Dentistry
Oncology
Authors
LÃvia B.A. Fontes, Débora dos S. Dias, Beatriz J.V. Aarestrup, Fernando M. Aarestrup, Ademar A. Da Silva Filho, José Otávio do Amaral Corrêa,