Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5553529 | Biomedicine & Pharmacotherapy | 2017 | 9 Pages |
ObjectiveTo compare two different imaging media, diffusion weighted imaging (DWI) with apparent diffusion coefficient (ADC) and Gd-EOB-DTPA-enhanced magnetic resonance imaging (MRI) with perfusion parameters Ktrans, Kep, and relative contrast enhancement index (RCEI), in assessing the liver function via ischemia/perfusion injury (IRI) + partial hepatectomy rat model.MethodsRats underwent 0, 30 and 60Â min of ischemia/reperfusion with 30% of hepatectomy before subjected to Gd-EOB-DTPA-enhanced MRI in addition to 99mTc-GSA scintigraphy. For 99mTc-GSA scintigraphy test, the receptor index LHL15, modified receptor index and the blood clearance index HH15 were recorded. Apparent diffusion coefficient (ADC) was evaluated by using both mono- and bi-exponential models, and perfusion parameters Ktrans, Kep, and RCEI were measured. Liver function is tested by measuring activity of serum ALT, AST and PT. Histological analysis was performed by H&E and Ki-67 staining.Results99mTc-GSA dynamic imaging analysis demonstrated that LHL15 was increased and HH15 was decreased as the extension of ischemia/reperfusion time. ADC value estimated by MRI was significantly increased (PÂ <Â 0.05) in 30Â min IRI group compared with 0Â min and 60Â min IRI groups, respectively. Ktrans value was gradually and significantly decreased (PÂ <Â 0.05) as the extension of IRI time, but there was no significant difference (PÂ >Â 0.05) in Kep value between at 30Â min and 60Â min IRI, and RCEI value was significantly higher (PÂ <Â 0.05) in 30Â min IR compared with 0Â min and 60Â min IRI group. Serum level of ALT, AST and PT were gradually and significantly (PÂ <Â 0.05) increased as the extension of IRI time. Histological analysis showed that there was a remarkable difference between 30Â min and 60Â min IRI, as protein expression of Ki-67 was significantly higher (PÂ <Â 0.05) in 30Â min IRI group.ConclusionFast ADC bi-exponential model in DWI and RCEI in Gd-EOB-DTPA-enhanced MRI showed the good correlation in assessment of liver function after partial hepatectomy, showing consistency with our histological findings. The Ktrans in Gd-EOB-DTPA-enhanced MRI could be a potent parameter for assessing the early ischemic injury, but not the severity of the hepatic injury, in accordance with the correlation with our biochemical findings.