Article ID Journal Published Year Pages File Type
5554844 European Journal of Pharmacology 2016 6 Pages PDF
Abstract

Increasing evidence indicates that the dysregulation of microRNAs is involved in tumor progression and development. The purpose of the present study was to explore the expression of microRNA-132 (miR-132) and its function in laryngeal squamous cell carcinoma (LSCC). The results showed that miR-132 expression was markedly upregulated in LSCC tissues and cell lines. Functional analyses indicated that overexpression of miR-132 enhanced cell proliferation and tumor growth, which resulted in the downregulation of p27 and p21 and the upregulation of cyclin D1. In addition, luciferase activity indicated that miR-132 directly targets FOXO1, and inhibits FOXO1 protein expression in LSCC cells. Further studies revealed that the ectopic expression of FOXO1 effectively reversed the cell growth induced by miR-132. Moreover, miR-132 also activated the PI3K/AKT pathway, which further decreased FOXO1 expression. In conclusion, these findings demonstrated that miR-132 plays an important oncogenic role in LSCC by modulating the PI3K/AKT/FOXO1 pathway at multiple levels, resulting in strong prognostic implication. Therefore, miR-132 might be a potential therapeutic strategy in LSCC.

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