Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5555441 | International Immunopharmacology | 2017 | 6 Pages |
â¢SchB inhibited the production of TNF-α and IL-8 in HUVECs stimulated with LPS.â¢SchB also inhibited LPS-induced VCAM-1 and ICAM-1 expression.â¢SchB blocked the activation of NF-κB induced by LPS.â¢SchB increased the expression of Nrf2 and HO-1 in a concentration-dependent manner.â¢The inhibition of TNF-α and IL-8 production by SchB was blocked by transfection with Nrf2 siRNA.
Schisandrin B (SchB), an active ingredient extracted from Schisandra chinensis (Turcz.) Baill, has been known to have anti-oxidant and anti-inflammatory activities. In this study, we investigated the anti-inflammatory effects and mechanism of SchB in LPS-stimulated human umbilical vein endothelial cells (HUVECs). The effects of SchB on VCAM-1, ICAM-1, NF-κB and Nrf2 expression were detected by western blot analysis. The effects of SchB on TNF-α and IL-8 production were detected by ELISA. The results showed that SchB strongly suppressed the production of TNF-α and IL-8 in HUVECs stimulated with LPS. SchB also inhibited LPS-induced VCAM-1 and ICAM-1 expression. Furthermore, SchB blocked the activation of NF-κB induced by LPS. In addition, SchB increased the expression of Nrf2 and HO-1 in a concentration-dependent manner. And the inhibition of TNF-α and IL-8 production by SchB was blocked by transfection with Nrf2 siRNA. Our findings showed that SchB inhibited LPS-induced inflammation in HUVECs by activating Nrf2 signaling pathway.