Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5561616 | Reproductive Toxicology | 2016 | 7 Pages |
Abstract
As bisphenol A (BPA) exerts oxidative/pro-apoptotic effects in several cell types, we explored whether the in vitro exposure to BPA could affect human sperm integrity through the induction of pro-oxidative/apoptotic mitochondrial dysfunction. The exposure of motile sperm suspensions to scalar BPA concentrations for 4 h produced a decrease in the mitochondrial membrane potential, starting from 300 μM. It was associated with an increased mitochondrial generation of superoxide anion, caspase-9 and caspase-3 activation and motility decrement. Vitality decline was observed at BPA â¥Â 400 μM. Twenty hours exposure to 300 μM BPA, but not to lower concentrations, produced a significant loss in sperm vitality associated with a complete sperm immobilization. Finally, 300 μM BPA also produced a significant DNA oxidative damage, as revealed by the formation of oxidized base adduct 8-hydroxy-2â²-deoxyguanosine. In conclusion, BPA affected human sperm integrity by inducing pro-oxidative/apoptotic mitochondrial dysfunction.
Keywords
BPAVCLstraight line velocityBWWVSLVAP8OHdGΔΨmMSRPBSFITC8-hydroxy-2′-deoxyguanosineDMSOROSDNA damageBisphenol Asperm motilityCASAApoptosisDimethylsulfoxideSperm viabilityCurvilinear velocityfluorescein isothiocyanateFlow cytometryPhosphate-buffered salineMitochondrial membrane potentialcaspaseReactive oxygen species
Related Topics
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Authors
Arcangelo M.D., Chiara Castellini, Noemi Di Giammarco, Gaetano Santilli, Sandro Francavilla, Felice Francavilla,