Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5562522 | Toxicology in Vitro | 2017 | 12 Pages |
Abstract
Hand hygiene plays a key role in nosocomial infection prevention. To achieve users' adherence, products' dermal tolerance is essential. We aimed at making a comparative assessment of skin irritation and phototoxicity of the 3 alcohols commonly used in alcohol-based hand rubs (Ethanol, Propan-2-ol, Propan-1-ol) at 60, 70, 80 or 85% w/w in water or with co-formulates (hydrating, emollient and skin protective agents). In vitro validated OECD methods 439 and 432 were used. For irritation, EpiSkin⢠Small Model was the chosen Reconstructed Human Epidermis (RhE). For phototoxicity, co-formulates alone or in mixture with and without alcohol were tested using BALB/c 3T3 cell cultures. Whilst Ethanol and Propan-2-ol could not be differentiated and displayed good skin tolerance profiles, Propan-1-ol based products lead to significant viability impairments of RhE at 60, 70 or 80% and at 60% in the presence of co-formulates. However, these results could not be reproduced in another RhE model. Taking also into account bibliographic data on Propan-1-ol, this suggests that our results are probably related to a lack of specificity of the used RhE. Therefore, it can be relevant in case of significant results to use two different RhE models before performing any classification and/or performing any complementary tests.
Keywords
Alcohol-based hand rubsEFSACOLIPAOECDESACATCCSCCsGHSECVAMECVAM Scientific Advisory CommitteeEuropean Food Safety AuthorityReconstructed human epidermisHand hygieneSkin irritationIn vitroWorld Health OrganizationUnited NationsOrganization for Economic Co-operation and DevelopmentGlobally Harmonized SystemPhototoxicityAmerican Type Culture CollectionEuropean Centre for the Validation of Alternative MethodsScientific Committee on Consumer SafetyWHO
Related Topics
Life Sciences
Environmental Science
Health, Toxicology and Mutagenesis
Authors
Monique Manche, Benoît Foligné, Mathieu Sauty, Anne Platel, Eric Vercauteren, Gaétan Rauwel, Sophie Catoire, Hervé Ficheux, Jacques Criquelion, Fabrice Nesslany,