Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5562582 | Toxicology in Vitro | 2017 | 22 Pages |
Abstract
The present study aimed to investigate whether MBZ down-regulates drug transporter expression (ABCB1, ABCC1, SLC47A1). mRNA expression level of ABCB1, ABCC1 and SLC47A1 was evaluated by qPCR and protein expression levels MDR-1 was performed by western blotting in malignant ascites cells (AGP-01) treated with MBZ for 24 h. The mRNA expression level of ABCB1 and ABCC1 significantly decreased at a 1.0 μM of MBZ compared to negative control, while SLC47A1 extremely decreased at all tested concentrations of MBZ. Protein expression levels MDR-1 significantly decreased at a 1.0 μM of MBZ compared to negative control. Therefore, our results showed MBZ may play an important role in inhibiting MDR gene expression in malignant ascites cells.
Related Topics
Life Sciences
Environmental Science
Health, Toxicology and Mutagenesis
Authors
Laine Celestino Pinto, Caroline de Fátima Aquino Moreira-Nunes, Bruno Moreira Soares, Rommel Mário Rodriguez Burbano, José Alexandre Rodrigues de Lemos, Raquel Carvalho Montenegro,