| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5562726 | Toxicology in Vitro | 2017 | 9 Pages |
â¢Lipid-core nanocapsules (LNC) were coated with chitosan (CS).â¢Toxicity of LNC and LNC-CS after incubation with bovine sperm in different concentrationsâ¢CASA parameters and flow cytometry assays were performed to assess LNC and LNC-CS effects.â¢There is no toxicity from LNCs and LNC-CS above 40% (v/v) concentration.
Toxicology studies have a pivotal role for selection of new nanosystems. As lipid-core nanocapsules (LNC) rise as a potential system not only for drug delivery but also for immunotherapy and gene therapy, the demand for models of toxic screening increases, and sperm arises as a promising model due to the easiness to evaluate its viability parameters. LNCs were coated with chitosan, chitosan-coated lipid-core nanocapsules (LNC-CS), in order to modify the nanocapsule surface. We evaluated the toxicity of LNC and LNC-CS after incubation with bovine sperm in different concentrations (2.5%, 5%, 10%, 20%, 40% and 80%) (v/v) and periods of exposure (0Â h and 1Â h). CASA parameters and flow cytometry assays were performed to assess LNC and LNC-CS effects. The results corroborated with previous studies indicating that there is no toxicity from LNCs and LNC-CS below 40% (v/v) concentration.
