Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5585777 | International Journal of Developmental Neuroscience | 2017 | 40 Pages |
Abstract
Our data demonstrate that RELN, GAD1, and several other genes selectively expressed in GABAergic neurons, are down-regulated in post-mortem ASD FC. In addition, we observed increased DNMT1 and MECP2 binding at the corresponding promoters of these genes. The finding of increased MECP2 binding to the RELN, GAD1 and GAD2 promoters, with reduced amounts of 5mC and unchanged amounts of 5hmC present in these regions, suggests the possibility that DNMT1 interacts with and alters MECP2 binding properties to selected promoters. Comparisons between data obtained from the FC with CB studies showed some common themes between brain regions which are discussed.
Keywords
RELNGAD1DNA methyltransferase 1MeDIP5hmCReelinGABA neuronMecp2Gabrb3Dnmt1TETqRT-PCR5fC5caC5-carboxycytosineGAPDHGad25mC5-formylcytosine5-Methylcytosine5-hydroxymethylcytosineBDNFAutism spectrum disordergamma-aminobutyric acidOxtrchromatin immunoprecipitationconReSTGene expressionImmunoprecipitationRettRepressorsTranscriptionRett syndromebrain derived neurotrophic factorActbfrontal cortexMethylcytosineCerebellumBrodmann areaASDhydroxymethylcytosinequantitative reverse transcription polymerase chain reactionmethyl CpG binding protein 2CHiPChromatinGABAglutamate decarboxylase 65glutamate decarboxylase 67glyceraldehyde-3-phosphate dehydrogenaseoxytocin receptor
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Authors
Adrian Zhubi, Ying Chen, Alessandro Guidotti, Dennis R. Grayson,