Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5585829 | Journal of Genetics and Genomics | 2017 | 11 Pages |
Abstract
Reverse genetic screens are invaluable for uncovering gene functions, but are traditionally hampered by some technical limitations. Over the past few years, since the advent of the revolutionary CRISPR/Cas9 technology, its power in genome editing has been harnessed to overcome the traditional limitations in reverse genetic screens, with successes in various biological contexts. Here, we outline these CRISPR/Cas9-based screens, provide guidance on the design of effective screens and discuss the potential future directions of development of this field.
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Authors
Wanjing Shang, Fei Wang, Gaofeng Fan, Haopeng Wang,