Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5587654 | General and Comparative Endocrinology | 2017 | 9 Pages |
Abstract
Biologically active recombinant yellowtail kingfish follicle stimulating hormone (rytkFsh) was produced in yeast Pichia pastoris and its biological activity was demonstrated by both in-vitro and in-vivo bioassays. Incubation of ovarian and testicular fragments with the recombinant hormone stimulated E2 and 11-KT secretion, respectively. In-vivo trial in immature female YTK resulted in a significant increase of plasma E2 levels and development of oocytes. In males at the early stages of puberty, advancement of spermatogenesis was observed, however plasma 11-KT levels were reduced when administered with rytkFsh.
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Authors
Pablo J. SanchÃs-Benlloch, Josephine Nocillado, Claudia Ladisa, Joseph Aizen, Adam Miller, Michal Shpilman, Berta Levavi-Sivan, Tomer Ventura, Abigail Elizur,