Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5589149 | Gene | 2017 | 6 Pages |
Abstract
The purpose of our study was to identify new pathogenic genes used for exploring the pathogenesis of rheumatoid arthritis (RA). To screen pathogenic genes of RA, an integrated analysis was performed by using the microarray datasets in RA derived from the Gene Expression Omnibus (GEO) database. The functional annotation and potential pathways of differentially expressed genes (DEGs) were further discovered by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis. Afterwards, the integrated analysis of DNA methylation and gene expression profiling was used to screen crucial genes. In addition, we used RT-PCR and MSP to verify the expression levels and methylation status of these crucial genes in 20 synovial biopsy samples obtained from 10 RA model mice and 10 normal mice. BCL11B, CCDC88C, FCRLA and APOL6 were both up-regulated and hypomethylated in RA according to integrated analysis, RT-PCR and MSP verification. Four crucial genes (BCL11B, CCDC88C, FCRLA and APOL6) identified and analyzed in this study might be closely connected with the pathogenesis of RA.
Keywords
Pathogenic genesRMAGEOPTPN22HLA-DRFLSDEGsRheumatoid arthritisOsteoarthritisKEGG یا Kyoto Encyclopedia of Genes and Genomes Kyoto Encyclopedia of Genes and GenomesDAVIDfibroblast-like synoviocytesDNA methylationGene ontologyPathogenesisthe Database for Annotation, Visualization and Integrated DiscoveryGene Expression OmnibusDifferentially expressed genes
Related Topics
Life Sciences
Biochemistry, Genetics and Molecular Biology
Genetics
Authors
Lei Zhang, Shiyun Ma, Huailiang Wang, Hang Su, Ke Su, Longjie Li,