Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5589159 | Gene | 2017 | 8 Pages |
Abstract
MicroRNAs (miRNAs) are small non-coding RNAs (ncRNAs) that mainly bind to the seed sequences located within the 3â² untranslated region (3â² UTR) of target genes. They perform an important biological function as regulators of gene expression. Different genes can be regulated by the same miRNA, whilst different miRNAs can be regulated by the same genes. Here, the evolutionary conservation and expression pattern of miR-10a-3p in olive flounder and rock bream was examined. Binding sites (AAAUUC) to seed region of the 3â² UTR of target genes were highly conserved in various species. The expression pattern of miR-10a-3p was ubiquitous in the examined tissues, whilst its expression level was decreased in gill tissues infected by viral hemorrhagic septicemia virus (VHSV) compared to the normal control. In the case of rock bream, the spleen, kidney, and liver tissues showed dominant expression levels of miR-10a-3p. Only the liver tissues in the rock bream samples infected by the iridovirus indicated a dominant miR-10a-3p expression. The gene ontology (GO) analysis of predicted target genes for miR-10a-3p revealed that multiple genes are related to binding activity, catalytic activity, cell components as well as cellular and metabolic process. Overall the results imply that the miR-10a-3p could be used as a biomarker to detect VHSV infection in olive flounder and iridovirus infection in rock bream. In addition, the data provides fundamental information for further study of the complex interaction between miR-10a-3p and gene expression.
Keywords
CDC42epithelioma papulosum cyprinidRBIVC19MCPBXHOXVHSVADARIridovirushomeoboxMLLCFUGCSFncRNAsRT-PCREPCPPImiRNAshpi3′ UTR3′ untranslated regioncDNAComplementary DNAnon-coding RNAsStreptococcus parauberisExpressionBiomarkerProtein ANalysis THrough Evolutionary RelationshipsProtein-protein interactionGene–gene interactionRock bream iridovirusreverse transcriptionmicroRNAshours post infectionGranulocyte colony stimulating factornuclear factor (erythroid-derived 2)-like 2Evolutionmixed-lineage leukemiaMicroRNAGene ontologyHepatitis C virusHCVcolony forming unitsplaque-forming unitsreverse transcription polymerase chain reactionviral hemorrhagic septicemia virusPANTHERcell division control protein 42 homologpfu
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Authors
Ara Jo, Jennifer Im, Hee-Eun Lee, Dongmin Jang, Gyu-Hwi Nam, Anshuman Mishra, Woo-Jin Kim, Won Kim, Hee-Jae Cha, Heui-Soo Kim,