Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5589213 | Gene | 2017 | 27 Pages |
Abstract
Nuclear mitochondrial DNA sequences (NUMTs) have been documented in almost all eukaryotic genomes studied. Recently, with the number of sequenced genomes increasing, extremely large NUMTs, even a nearly entire mitochondrial genome, have been reported in some plants and animals. However, few such studies provided strong experimental evidences for these important discoveries. In this study using a computer-based search method an entire mitochondrial genome (NUMT-1) was found in the nuclear genome of a bat species (Myotis lucifugus). This super-large NUMT shared a same scaffold with a 754Â bp nuclear genomic sequence and a second NUMT (NUMT-2, 3292Â bp). If NUMT-1 was real, it will be the largest NUMT found in animals and this finding will provide valuable insights into the mode of generation of NUMTs in the nuclear genome. Unfortunately, although the initial sequencing technology of the published M. lucifugus genome makes the possibility of artifact less likely, our results from both the PCR amplification followed by Sanger sequencing and mapping method based on the whole-genome resequencing datasets suggested that the scaffold containing the entire mitochondrial genome was artifact possibly due to a misassembly of mitochondrial and the nuclear DNA sequences. Our current study highlights the necessity to validate the authenticity of extremely large NUMTs identified in previous searches on whole-genome sequence assemblies.
Keywords
atp6ATP synthase F0 subunit 8ATP synthase F0 subunit 6NADH dehydrogenase subunitND4LNADH dehydrogenase subunit 2NADH dehydrogenase subunit 1Cytochrome c oxidase subunit IIICOIIIND3ND1tRNAND2atp8cytBND6NADH dehydrogenase subunit 5ND5ND4Myotis lucifugusNADH dehydrogenase subunit 6NCBICytochrome c oxidase subunit ICOINumtSMitochondrial DNARibosomal RNArRNA, Ribosomal ribonucleic acidtransfer RNAmtDNAcytochrome c oxidase subunit IICytochrome bNational Center for Biotechnology InformationNADH dehydrogenase subunit 3COII
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Authors
Huizhen Shi, Yutong Xing, Xiuguang Mao,