Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5589570 | Gene | 2017 | 31 Pages |
Abstract
Adipose-derived stem cells (ASCs) isolated from subcutaneous (SC) and omentum (O) share similar characteristics, but the differences in their microRNA profiles are mostly unknown. In this study, besides significant differences in cell morphology and the differentiation ability of the two types of ASCs, the microRNA expression profiles of the cell lines were determined using SOLiD next-generation sequencing. The in-depth analysis found that miR-214, miR-222, miR-181a, miR-26a and miR-23/27/24 clusters and miR-375 act as “markers” to distinguish the different fat deposit-derived ASCs. Additionally, the global miRNA-mRNA interaction differences were revealed, and the results of the GO term enrichment and KEGG pathway in the DAVID tool showed that the molecular function, biological process and signaling pathways showed some different in the two types of ASCs. Our findings provided a clue to a more thorough understanding of the difference between SC-ASCs and O-ASCs and indicate their different potentials for clinical use.
Keywords
PBSOCNASCsCollagen type-IHoechst 33342FITCFBSDMEMBCIP/NBTDulbecco's modified Eagle MediumALPAlkaline phosphataseOsteocalcinBioinformaticssolidKEGG یا Kyoto Encyclopedia of Genes and Genomes Kyoto Encyclopedia of Genes and GenomesDAVIDfetal bovine serumAdipose-derived mesenchymal stem cellsphycoerythrinfluorescein isothiocyanatePhosphate-buffered salineGene ontologydatabase for annotation, visualization and integrated discoveryHOEcol
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Authors
Hu Feihu, Xu Peng, Sun Bo, Xiao Zhongdang,