Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5589788 | Gene | 2017 | 9 Pages |
â¢DLK1-DIO3 microRNA cluster has different expression between OCSCs and OCCs (CD44 â/CD133 â).â¢Low miR-134-3p in OCSCs correlates with RAB27a overexpression.â¢We confirmed RAB27a mRNA a novel target of miR-134-3p.â¢Overexpression for miR-134-3p inhibits OCSCs in vitro.â¢MiR-134-3p transfection decreases OCSCs xenograft tumor growth.
The cluster of microRNAs (miRNAs) in the DLK1-DIO3 genomic imprinted region contains several miRNAs that have a significant regulatory role in tumor proliferation and invasion. One of these miRNAs is miR-134-3p, and its expression changes significantly in human ovarian cancer stem cells (OCSCs) and in CD44Â â/CD133Â â ovarian cancer. The results of a luciferase assay showed that miR-134-3p silenced RAB27A by binding to the 3â²-UTR of RAB27A mRNA. Overexpression of miR-134-3p in human OCSCs can not only inhibit the expression of RAB27A but also can effectively downregulate the expression of some tumor proliferation and invasion genes. Overexpression of miR-134-3p can not only inhibit the in vitro proliferation and cell cycle progression of human OCSCs but also can decrease the tumorigenicity in nude mice.