Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5589849 | Gene | 2016 | 28 Pages |
Abstract
FAM3A (family with sequence similarity 3, member A) is regulated by PPARG and participates in the metabolism of lipid in liver. However, the transcriptional regulation analysis of FAM3A is very little and biological function of FAM3A still unclear. In this study, the core promoter region and transcription factor binding sites of FAM3A gene were identified and characterized using dual luciferase report experiments and electrophoretic mobility shift assays (EMSA). The promoter activity of FAM3A was dramatically decreased after the mutation of C/EBPβ binding sites, suggesting that C/EBPβ is a transcriptional activator of FAM3A. Overexpression of FAM3A significantly inhibited the efficiency of preadipocytes to differentiate into adipocytes as indicated by Western Blot and Oil Red O staining assay. These results suggest that C/EBPβ plays an important role in regulating FAM3A promoter activity and FAM3A inhibits adipocyte differentiation.
Keywords
FABP4hepatocyte nuclear factor 1ALPLHNF1AMDICREBUCP2CCAAT/enhancer binding protein betaSCD1HSLWnt10bACC2ACOX1acetyl-CoA carboxylase 2BATDGAT1FASRGZG6PDSREBP1FAM3Adiacylglycerol o-acyltransferase 1C/EBPαc/ebpβAdipogenesisStearoyl-CoA desaturase 1fatty acid synthasebrown adipose tissuecoding sequencerosiglitazoneLipoprotein lipaseCCAAT/enhancer binding protein alphaFatty acid-binding proteinSterol regulatory element binding protein 1uncoupling protein 2cAMP-responsive element-binding proteinPromoterCdSglucose-6-phosphate dehydrogenase
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Authors
Tingting Kang, Disha Peng, Guowei Bu, Hao Gu, Feng Zhang, Ruirui Zhang, Ying Zhou, Yuanzhu Xiong, Minggang Lei,