Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5589902 | Gene | 2017 | 31 Pages |
Abstract
To further supplement the genome-level features in related species, T. quinquevittatum complete mtDNA was firstly sequenced and de novo assembled by next-generation sequencing. The full-length mtDNA of T. quinquevittatum was a 16,896Â bp fragment, which was atypical of Labridae, with 2 ribosomal RNA (rRNA) genes, 13 protein-coding genes (PCGs), 23 transfer RNA (tRNA) genes, and a major non-coding control region (D-loop region). Additionally, the mtDNA of T. quinquevittatum exhibited characteristics of A (27.1%), T (29.3%), G (17.8%), and C (25.8%) with a high AÂ +Â T content (56.4%). Furthermore, the analysis of the average Ka/Ks in the 13 PCGs of three Labridae species indicated a strong purifying selection within this group. Additionally, the phylogenetic analysis based on 13 concatenated PCGs nucleotide and amino acid datasets, showed high value support for the following sister clade among the four genera (T. quinquevittatum, Halichoeres trimaculatus, Halichoeres melanurus, Parajulis poecilepterus). The complete mtDNA of the T. quinquevittatum provided important information for the study in population genetics and evolutionary theory.
Keywords
PCGsapocytochrome bCytochrome c oxidase subunits I–IIIATPase subunits 6 and 8rRNAND1–ND6 and ND4LD-loop regionKa/KstRNAcytBatp6 and atp8Mitochondrial DNARibosomal RNAtransfer RNAphylogenetic analysisBayesian inferenceMaximum likelihoodMaximum parsimonyNADH dehydrogenase subunits 1–6 and 4LProtein-coding genes
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Genetics
Authors
Kecheng Zhu, Na Wu, Xiaoxiao Sun, Huayang Guo, Nan Zhang, Shigui Jiang, Dianchang Zhang,