Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5591006 | Plasmid | 2017 | 22 Pages |
Abstract
Throughout the past decade, the field of synthetic biology has grown rapidly. By using assembly platforms such as BioBricksâ¢, scientists can quickly and easily build gene circuits or multi-step pathways. One limitation, however, is that most of these parts were designed and characterized with Escherichia coli as the target chassis. As a consequence, there exists a lack of standardized and well characterized or BioBrick⢠compatible plasmid backbones that replicate in other potential non-model chassis organisms. The Gram-positive bacteria of the genus Rhodococcus represent an interesting chassis for biotechnological applications due to their tremendous metabolic capabilities. In this report we describe our progress toward developing a BioBrick⢠compatible plasmid system for Rhodococcus. We demonstrate its utility for heterologous protein expression through flow cytometric analysis of the lac promoter in the oleaginous strain Rhodococcus opacus PD630.
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Authors
James Ellinger, Claudia Schmidt-Dannert,