Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5591488 | Journal of Structural Biology | 2017 | 13 Pages |
Abstract
We have developed a software package towards automatic electron tomography (ET): Automatic Tomography (AuTom). The presented package has the following characteristics: accurate alignment modules for marker-free datasets containing substantial biological structures; fully automatic alignment modules for datasets with fiducial markers; wide coverage of reconstruction methods including a new iterative method based on the compressed-sensing theory that suppresses the “missing wedge” effect; and multi-platform acceleration solutions that support faster iterative algebraic reconstruction. AuTom aims to achieve fully automatic alignment and reconstruction for electron tomography and has already been successful for a variety of datasets. AuTom also offers user-friendly interface and auxiliary designs for file management and workflow management, in which fiducial marker-based datasets and marker-free datasets are addressed with totally different subprocesses. With all of these features, AuTom can serve as a convenient and effective tool for processing in electron tomography.
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Authors
Renmin Han, Xiaohua Wan, Zihao Wang, Yu Hao, Jingrong Zhang, Yu Chen, Xin Gao, Zhiyong Liu, Fei Ren, Fei Sun, Fa Zhang,