| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5618789 | Molecular Metabolism | 2017 | 9 Pages |
Abstract
We successfully derived a Gcg-CreERT2 mouse line that expresses CreERT2 in pancreatic α-cells and enteroendocrine L-cells without disrupting preproglucagon gene expression. These mice will be a useful tool for performing temporally controlled genetic manipulation specifically in these cell types.
Keywords
FACSYFPIRESLSLGLP1DAPIGCGCreERT2gRNACRECRISPR4′,6-diamidino-2-phenylindoleα-Cellclustered regularly interspaced short palindromic repeatIsletESCfluorescence-activated cell sortingguide RNAinternal ribosomal entry siteEmbryonic stem cellUTR یا untranslated regions untranslated regionyellow fluorescent proteinglucagon-like peptide 1Cre recombinaseGlucagon
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Authors
Amanda M. Ackermann, Jia Zhang, Aryel Heller, Anna Briker, Klaus H. Kaestner,