Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5665861 | Diagnostic Microbiology and Infectious Disease | 2017 | 4 Pages |
Abstract
â¢We developed a multiplex real-time PCR assay for detecting and typing M. pneumoniae.â¢Typing was based on the presence of short type-specific indels for P1 typing.â¢This assay was 100% specific compared to existing methods.
We developed a multiplex real-time PCR assay for simultaneously detecting M. pneumoniae and typing into historically-defined P1 types. Typing was achieved based on the presence of short type-specific indels identified through whole genome sequencing. This assay was 100% specific compared to existing methods and may be useful during epidemiologic investigations.
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Authors
Bernard J. Wolff, Alvaro J. Benitez, Heta P. Desai, Shatavia S. Morrison, Maureen H. Diaz, Jonas M. Winchell,