Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5672798 | Journal of Infection and Public Health | 2017 | 8 Pages |
Abstract
In this study, we assessed the accuracy in identification of both Gram-negative and Gram-positive bacteria, except for Streptococcus viridans, using four rapid blood culture methods with Vitek MALDI-TOF-MS. We compared our proposed lysis centrifugation followed by washing and 30% acetic acid treatment method (method 2) with two other lysis centrifugation methods (washing and 30% formic acid treatment (method 1); 100% ethanol treatment (method 3)), and picking colonies from 90 to 180Â min subculture plates (method 4). Methods 1 and 2 identified all organisms down to species level with 100% accuracy, except for Streptococcus viridans, Streptococcus pyogenes, Enterobacter cloacae and Proteus vulgaris. The latter two were identified to genus level with 100% accuracy. Each method exhibited excellent accuracy and precision in terms of identification to genus level with certain limitations.
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Authors
Ali M. Bazzi, Ali A. Rabaan, Zeyad El Edaily, Susan John, Mahmoud M. Fawarah, Jaffar A. Al-Tawfiq,