| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 5672901 | Journal of Virological Methods | 2017 | 9 Pages |
â¢A novel method to identify Hepatitis C Virus (HCV) host interactors in the HCV cell culture system was developedâ¢An inducible stable trans-encapsidation system was optimized to allow temporal separation in HCV life cycleâ¢A transient trans-encapsidation system was optimized for biosafe live imaging of HCV NS5A and core viral proteins
Proteomics and imaging techniques are used more and more in tandem to investigate the virus-host interaction. Herein we present novel replicons, methods and trans-encapsidation systems suitable for determination of Hepatitis C Virus (HCV) proteins interactomes and live imaging of viral proteins dynamics in HCV cell culture (HCVcc) system. To identify endogenous factors involved in the HCV life cycle, we constructed full-length functional replicons with affinity purification (AP) tags fused to NS2 and NS5A proteins. Viral-host interactomes were determined and validated in HCVcc system. To investigate the dynamics of viral-host interactions, we developed a core-inducible packaging cell line which trans-encapsidates various subgenomic replicons suitable for AP in replication and assembly stages. Further, a transient trans-encapsidation system was developed for live imaging of the NS5A viral protein in replication and assembly steps, respectively. The NS5A dynamics was determined also in the full-length HCV replicon system. The analysis of NS5A dynamics showed a decreased mobility of the protein in assembly versus the replication step. The tools presented herein will allow the investigation of HCV-host interaction with improved biological relevance and biosafety.
