Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
5672980 | Journal of Virological Methods | 2017 | 6 Pages |
â¢We generated two defective bovine parainfluenza virus type 3 strains.â¢Two defective viruses could be maintained in cell cultures by co-infection.â¢This method would be useful to express large or multiple proteins in cell cultures.
Two defective bovine parainfluenza virus type 3 (BPIV3) strains were generated, one lacking the membrane (M) protein gene and expressing EGFP (ÎM-EGFP) and the other lacking the fusion (F) protein gene and expressing mStrawberry (ÎF-mSB), by supplying deficient proteins in trans. When Madin-Darby bovine kidney (MDBK) cells were co-infected with ÎM-EGFP and ÎF-mSB at a multiplicity of infection (MOI) of 0.1, complemented viruses were easily obtained. Complemented viruses grew as efficiently as wild-type BPIV3 and could be passaged in MDBK cell cultures even at an MOI of 0.01, possibly due to multiploid virus particles containing genomes of both ÎM-EGFP and ÎF-mSB. This reciprocal complementation method using two defective viruses would be useful to express large or multiple proteins in cell cultures using paramyxovirus vectors.